Journal: Frontiers in Immunology

Article Title: The APC/C subunit APC7 exerts antiviral effects by targeting the adaptor protein MAVS

doi: 10.3389/fimmu.2026.1912055

Figure Lengend Snippet: Overexpression of APC7 promotes RNA virus-induced innate immune response. (A) HeLa cells were transfected with empty vector or HA-APC7 plasmids for 24 h and infected with SeV (MOI = 1) or transfected with poly(I:C) (3 μg/ml) for another 16 h. The mRNA levels of IFN-β and ISG56 were determined by qPCR. (B) HeLa cells were transfected with empty vector or HA-APC7 plasmids for 24 h and then infected with SeV (MOI = 1) for 0, 9, and 12 h. The expression of various proteins in the cell lysates was analyzed with the indicated antibodies, and the relative levels of pTBK1/TBK1, pIRF3/IRF3 and SeV/GAPDH were quantified by ImageJ. (C, D) HeLa cells were transfected with empty vector or HA-APC7 plasmids for 24 h and then infected with SeV (MOI = 1) for 12 h. The cells were lysed to prepare cytoplasmic and nuclear fractions, and the levels of various proteins were determined by Western blotting (C) . The levels of IRF3 relative to GAPDH or Lamin B1 were quantified by ImageJ. The expression of IRF3 (red) in the nuclei (blue) was detected by confocal microscopy (D) . Bar = 50 μm. The graphs are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The double-stranded RNA (dsRNA) analog poly(I:C) (tlrl-pic) was obtained from InvivoGen (San Diego, CA, USA).

Techniques: Over Expression, Virus, Transfection, Plasmid Preparation, Infection, Expressing, Western Blot, Confocal Microscopy

Journal: Frontiers in Immunology

Article Title: The APC/C subunit APC7 exerts antiviral effects by targeting the adaptor protein MAVS

doi: 10.3389/fimmu.2026.1912055

Figure Lengend Snippet: Knockdown of APC7 reduces RNA virus-triggered innate immune response. (A) HeLa cells were transfected with siNC or siAPC7 for 24 h and then infected with VSV or SeV at an MOI of 1, or transfected with poly(I:C) (3 μg/ml) for 16 h. The mRNA levels of IFN-β and ISG56 were determined by qPCR. (B) THP-1 cells stably expressing shNC or shAPC7 were generated and infected with VSV or SeV at an MOI of 1, or transfected with poly(I:C) (3 μg/ml) for 12 h. The mRNA levels of IFN-β and ISG56 were determined by qPCR. (C) HeLa cells were transfected with siNC or siAPC7 for 24 h and then infected with SeV (MOI = 1) for the indicated time. The levels of various proteins in the cell lysates were detected by Western blot analysis; the relative expression levels of pTBK1/TBK1, pIRF3/IRF3 and SeV/GAPDH were quantified by ImageJ. (D, E) HeLa cells were transfected with siNC or siAPC7 for 24 h and then infected with SeV (MOI = 1) for 12 h. The cells were lysed to prepare cytoplasmic and nuclear fractions, and the levels of various proteins were determined by immunoblotting (D) . The levels of IRF3 relative to GAPDH or Lamin B1 were quantified by ImageJ. The expression of IRF3 (red) in the nuclei (blue) was detected by confocal microscopy (E) . Bar = 50 μm. The graphs are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The double-stranded RNA (dsRNA) analog poly(I:C) (tlrl-pic) was obtained from InvivoGen (San Diego, CA, USA).

Techniques: Knockdown, Virus, Transfection, Infection, Stable Transfection, Expressing, Generated, Western Blot, Confocal Microscopy